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KMID : 1137820130340030123
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의공학회지
2013 Volume.34 No. 3 p.123 ~ p.128
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Investigation of the Binding Force between Protein A and Immunoglobulin G Using Dielectrophoretic(DEP) Tweezers Inside a Microfluidic Chip
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Kwak Tae-Joon
Lee Jae-Woo
Yoon Dae-Sung
Lee Sang-Woo
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Abstract
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The ’Dielectrophoretic Tweezers(DEP Tweezers)’ can be used as a facile, economical toolkit for quantitative measurement of chemical and biological binding forces related to many biological interactions within a microfluidic device. Our experimental setup can probe the interaction between a single receptor molecule and its specific ligand. mmunoglobulin G(IgG) functionalized on polystyrene microspheres has been used to detect individual surface linked Staphylococcus protein A(SpA) molecules and to characterize the strength of the noncovalent IgG-SpA bond. It was measured and compared with the existing measurements. Measured single binding force of between Goat, Rabbit IgG and SpA were 17 ± 7pN, 74 ± 16pN. This work can be used to investigate several different ligand-receptor interactions and antigen-antibody interactions.
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KEYWORD
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Dielectrophoresis, Force Spectroscopy, Intermolecular interactions, Immunoglobulin G, Protein A
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